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purelink hipure plasmid filter maxiprep kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher purelink hipure plasmid filter maxiprep kit
    Purelink Hipure Plasmid Filter Maxiprep Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purelink+maxiprep+kit/purelink+rna+mini+kit/pm40639723-52-7-15
    Average 90 stars, based on 1 article reviews
    purelink hipure plasmid filter maxiprep kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Posttranslationally modified self-peptides promote hypertension in mouse models
    Article Snippet: .. Resultant vectors were transformed into ampicillin-treated E. coli (DH5α), purified using the PureLink Maxiprep kit (Thermo Fisher Scientific), and sequenced to confirm the insertion. ..

    Article Title: Discovery of post-translationally modified self-peptides that promote hypertension
    Article Snippet: .. Resultant vectors were transformed into ampicillin-treated E. Coli (DH5α), purified using the ThermoFisher PureLink Maxiprep kit, and sequenced to confirmed insertion. ..

    Article Title: Posttranslationally modified self-peptides promote hypertension in mouse models
    Article Snippet: .. Resultant vectors were transformed into ampicillin-treated E . coli (DH5α), purified using the PureLink Maxiprep kit (Thermo Fisher Scientific), and sequenced to confirm the insertion. ..

    Purification:

    Article Title: Posttranslationally modified self-peptides promote hypertension in mouse models
    Article Snippet: .. Resultant vectors were transformed into ampicillin-treated E. coli (DH5α), purified using the PureLink Maxiprep kit (Thermo Fisher Scientific), and sequenced to confirm the insertion. ..

    Article Title: Discovery of post-translationally modified self-peptides that promote hypertension
    Article Snippet: .. Resultant vectors were transformed into ampicillin-treated E. Coli (DH5α), purified using the ThermoFisher PureLink Maxiprep kit, and sequenced to confirmed insertion. ..

    Article Title: Posttranslationally modified self-peptides promote hypertension in mouse models
    Article Snippet: .. Resultant vectors were transformed into ampicillin-treated E . coli (DH5α), purified using the PureLink Maxiprep kit (Thermo Fisher Scientific), and sequenced to confirm the insertion. ..

    Transfection:

    Article Title: Systemic RNA Interference Deficiency-1 (SID-1) Extracellular Domain Selectively Binds Long Double-stranded RNA and Is Required for RNA Transport by SID-1
    Article Snippet: .. Plasmids used for transfection were prepared from Escherichia coli using the PureLink Maxiprep kit (Life Technologies, Inc.). ..

    Article Title: ALS-linked mutations impair UBQLN2 stress-induced biomolecular condensate assembly in cells.
    Article Snippet: Patient-derived ALS-linked mutations (A282V, M446R, P497H, P497S, and P506T) were generated from UBQLN2 in a pmCherry-C1 vector using a Phusion Site-Directed Mutagenesis Kit (Thermo Scientific; cat. no. F541, year of purchase 2018). .. Transfection-grade plasmid DNA were isolated using a PureLink Maxiprep Kit (ThermoFisher; cat. no. K210016, year of purchase 2018). .. A U2OS cell line (kindly provided by the J. Paul Taylor lab, St. Jude Children’s Research Hospital) stably expressing G3BP1-GFP, a fluorescently tagged stress granule marker, was used for live-cell imaging experiments unless otherwise noted, as well as for fixed-cell experiments.

    Article Title: Selection of Apoptotic Cell Specific Human Antibodies from Adult Bone Marrow
    Article Snippet: For this purpose, the p2–20 VH and VL encoding genes were separately amplified by specific primers that added 5′ Age I and 3′ Xho I restriction sites, and PCR products were purified by QIAExII gel purification (Qiagen), cleaved by Age I and Xho I (New England Biolabs), and then repurified with Qiaquick purification kit (Qiagen) prior to ligation into cleaved and purified H and L chain vectors, respectively, with T4 ligase. .. Plasmids for transfections were prepared using Purelink Maxiprep kit (Invitrogen) following the manufacturer's instructions. .. HEK293T cells expressing a stably transfected human J-chain were cultured until 80% confluent in DMEM with 10% low IgG FBS with 1 mM sodium pyruvate, penicillin, streptomycin, glutamine (Invitrogen).

    Plasmid Preparation:

    Article Title: ALS-linked mutations impair UBQLN2 stress-induced biomolecular condensate assembly in cells.
    Article Snippet: Patient-derived ALS-linked mutations (A282V, M446R, P497H, P497S, and P506T) were generated from UBQLN2 in a pmCherry-C1 vector using a Phusion Site-Directed Mutagenesis Kit (Thermo Scientific; cat. no. F541, year of purchase 2018). .. Transfection-grade plasmid DNA were isolated using a PureLink Maxiprep Kit (ThermoFisher; cat. no. K210016, year of purchase 2018). .. A U2OS cell line (kindly provided by the J. Paul Taylor lab, St. Jude Children’s Research Hospital) stably expressing G3BP1-GFP, a fluorescently tagged stress granule marker, was used for live-cell imaging experiments unless otherwise noted, as well as for fixed-cell experiments.

    Article Title: A comprehensive genetic catalog of human double-strand break repair
    Article Snippet: .. After plasmid maxiprep (PureLink Maxiprep kit, Invitrogen, #K210007), original and amplified TKOv3 libraries were Illumina-sequenced to verify that sgRNA representation was maintained. .. To generate lentiviral particles, HEK293T cells were transfected with an equimolar mixture of the lentiviral library DNA plus the packaging capsids psPax2 and pMD2.G using GeneJuice (Sigma-Aldrich, #70967).

    Article Title: Multiple tolerance checkpoints restrain affinity maturation of B cells expressing the germline precursor of a lupus patient-derived anti-dsDNA antibody in knock-in mice.
    Article Snippet: .. To prepare unmethylated CpG motif-containing ssDNA, plasmid DNA [pcDNA3 (Invitrogen)] was prepared using a PureLink Maxiprep Kit (Thermo Fisher) and linearized by EcoRI (Takara). .. The linearized plasmid DNA was phenol/ chloroform-extracted and treated with Endotoxin Removal Solution (Sigma) according to the manufacturer’s protocol.

    Isolation:

    Article Title: ALS-linked mutations impair UBQLN2 stress-induced biomolecular condensate assembly in cells.
    Article Snippet: Patient-derived ALS-linked mutations (A282V, M446R, P497H, P497S, and P506T) were generated from UBQLN2 in a pmCherry-C1 vector using a Phusion Site-Directed Mutagenesis Kit (Thermo Scientific; cat. no. F541, year of purchase 2018). .. Transfection-grade plasmid DNA were isolated using a PureLink Maxiprep Kit (ThermoFisher; cat. no. K210016, year of purchase 2018). .. A U2OS cell line (kindly provided by the J. Paul Taylor lab, St. Jude Children’s Research Hospital) stably expressing G3BP1-GFP, a fluorescently tagged stress granule marker, was used for live-cell imaging experiments unless otherwise noted, as well as for fixed-cell experiments.

    Amplification:

    Article Title: A comprehensive genetic catalog of human double-strand break repair
    Article Snippet: .. After plasmid maxiprep (PureLink Maxiprep kit, Invitrogen, #K210007), original and amplified TKOv3 libraries were Illumina-sequenced to verify that sgRNA representation was maintained. .. To generate lentiviral particles, HEK293T cells were transfected with an equimolar mixture of the lentiviral library DNA plus the packaging capsids psPax2 and pMD2.G using GeneJuice (Sigma-Aldrich, #70967).



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